Table 1 Transformation Results
From: Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits
Amplified Insert Assembly | ||||||||
|---|---|---|---|---|---|---|---|---|
# | Ligation Inputs | Results | ||||||
| Ā | Insert | Vector | Ratio | Color | Correct | Incorrect | Background | % Accuracy |
| Ā | Ptet | lacZ Ī± | 1:1 | Blue | 819 | 6 | 1 | 99.6 Ā± 0.9% |
1 | Ptet | lacZ Ī± | 2:1 | Blue | 868 | 13 | 1 | 98.4 Ā± 0.7% |
| Ā | Ptet | lacZ Ī± | 4:1 | Blue | 793 | 0 | 1 | 99.9 Ā± 0.2% |
| Ā | PJ23100 | lacZ Ī± | 1:1 | Blue | 778 | 3 | 3 | 99.2 Ā± 0.6% |
2 | PJ23100 | lacZ Ī± | 2:1 | Blue | 1454 | 5 | 1 | 99.6 Ā± 0.8% |
| Ā | PJ23100 | lacZ Ī± | 4:1 | Blue | 607 | 0 | 1 | 99.8 Ā± 0.3% |
| Ā | lacZ Ī± | PJ23100 | 1:1 | Blue | 391 | 12 | 6 | 95.6 Ā± 2.2% |
3 | lacZ Ī± | PJ23100 | 2:1 | Blue | 547 | 2 | 3 | 99.1 Ā± 0.7% |
| Ā | lacZ Ī± | PJ23100 | 4:1 | Blue | 465 | 5 | 1 | 98.7 Ā± 3.3% |
| Ā | mRFP Driver | lacZ Ī± | 1:1 | Red | 676 | 34 | 5 | 94.6 Ā± 3.4% |
4 | mRFP Driver | lacZ Ī± | 2:1 | Red | 1329 | 24 | 1 | 98.2 Ā± 0.9% |
| Ā | mRFP Driver | lacZ Ī± | 4:1 | Red | 1761 | 22 | 6 | 98.4 Ā± 1.0% |
Three-Antibiotic Assembly | ||||||||
# | Ligation Inputs | Results | ||||||
| Ā | Prefix | Suffix | Vector | Color | Correct | Incorrect | % Accuracy | |
5 | PJ23100 | lacZ Ī± | pSB1C3 | Blue | 8 | 1 | 89% | |
5* | PJ23100 | lacZ Ī± | pSB1C3 | Blue | 418 | 43 | 90.7 Ā± 0.6% | |
6 | lacZ Ī± | mRFP Driver | pSB1C3 | Red | 3 | 1 | 75% | |
6* | lacZ Ī± | mRFP Driver | pSB1C3 | Red | 174 | 29 | 85.7 Ā± 4.6% | |