Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits

Table 3 Parts and Plasmids

Part#	Description	Size (bp)	Use	Plasmid	Resistance
BBa_I732018	lacZ α with a strong ribosome binding site	534	Insert	pSB1AK3	Ampicillin, Kanamycin
BBa_J23100	Strong constitutive promoter in mRFP testing device	35 + 890	Insert	pBca1020	Ampicillin
BBa_R0040	TetR repressible promoter	54	Insert	pSB1A2	Ampicillin
BBa_I732018	lacZ α with a strong ribosome binding site	534	Vector	pSB1C3	Chloramphenicol
BBa_J23100	Strong constitutive promoter in mRFP testing device	35 + 890	Vector	pSB1C3	Chloramphenicol

The plasmids used are described according to the parts registry number http://www.partsregistry.org and the size of the BioBrick part contained on the plasmid. Parts given two sizes are promoters contained on promoter testing devices that have an mRFP coding sequence between the SpeI and PstI sites. The sizes are given as the promoter and mRFP gene respectively.

ISSN: 1754-1611