Figure 2

Nucleotide sequences of the Bacillus BioBrick Box promoter collection. Sequences are aligned according to their -35 and -10 region. All promoter fragments were designed with 3′-end exactly upstream of the RBS. The -10 and -35 regions (if conserved) are shown in bold and underlined. Regulator binding sites for XylR in P _xylA and LiaR in P _liaI are shown in italics and underlined. The native P _xylA also contains a catabolite responsive element (CRE) that suppresses the transcription in the presence of glucose [53]. This binding site is downstream of the promoter and is not present in the P _xylA –fragment used here, which is derived from the pXT vector [39].