Figure 4

Filling height as a function of the relative perimeter. The liquid height is measured vertically at the center of the well bottom with the modified BioLector measurement system at a total liquid filling volume of 500 μL. The dashed line represents the transition from in-phase to out-of-phase liquid behavior. The perimeters of the different well geometries are related to the perimeter of the round geometry with the same well cross-section area. Shaking diameter: 3 mm, temperature: 25°C, shaking frequency: 400 – 1000 rpm.