Figure 6
From: Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering
Screening of the final plasmid with restriction enzymes. Illustration of the final plasmid is shown. For screening, the plasmid was cut with the BsiwI enzyme generating two fragments of 4.8Â kb and 0.8Â kb in size. AMP: Ampicillin resistance gene; PRE: posttranscriptional regulatory element; MPSV: myeloproliferative sarcoma virus promoter.