Fig. 3

A chemical cocktail for cyanobacterial lysis. All reactions were shaken for 90 min at 37 °C. a Testing the contribution of EDTA, cellulase, spermine, and DTT to cell lysis. All samples contained T4 lysozyme at 10 μg/mL and the detergents C7BzO (0.1 % w/v) and SB3-14 (1 % w/v). The “full cocktail” also contained EDTA at 1 mM, T. viride cellulase at 50 μg/mL, spermine at 40 μM, and DTT at 200 mM; other samples omitted one of the reagents as indicated. Error bars are standard deviations of three independent experiments. b A design-of-experiments optimization of the levels of lysozyme, detergent, spermine, and DTT. The lysozyme was cyanophage lysozyme in this experiment. “1 X detergents” refers to 1 % C7BzO and 0.1 % SB3-14 (w/v). Error bars are standard deviations of triplicate reactions. c Influence of DTT concentration with other components as described for the “full cocktail” in (A), omitting EDTA and cellulase. Error bars are standard deviations of three independent experiments