Table 1 Comparison of methods used to study effect of UV irradiation induced oxidative stress
MTT assay | Fluorescent assay | Electrochemical detection | |
|---|---|---|---|
Labeling | Yes | Yes | No |
Real time | NO | NO | Yes |
Sample consumption | Large | Large | Small |
Assay time | 24–72 h | 1-2 h | Within minutes |
Overall comparison | Alive cell catalyse enzyme to indirectly characterize cell growth. Evaluate the overall effect of treatment on cell survival, growth. | Fluorophore probe is used to label target molecules. Not feasibly for real-time characterizing a reaction. Staining process will affect the health of cells. | In site characterize a reaction. No damage to cells. Fast reaction time allows for high throughput screening. No expensive equipment is involved. |