Fig. 4

Immunohistochemical assessment of vascularization by vessel formation, and functional vascular endothelial cell count 28 days after myocardial infarction reperfusion injury. a images of CD31 and Alexa Fluor 488 (green), VWF and Alexa Fluor 594 (red), and DAPI (blue). Functional vascular cells were characteristic of CD31+ and VWF+. Scale bar represents 100 μm. Quantification of immunohistochemical assessment of vascularization by b functional vascular endothelial cell counts and c vessel counts with functional vascular endothelial cells. n = 7–12, error bars represent standard error of the mean and * indicates p < 0.05