Fig. 5
The dynamic range of four different arabinose-inducible reporter constructs, integrated sequentially into the genome of E. coli in four phage attachment sites (attHK022, attP21, attϕ80, attλ) and control strains in which the same reporter systems are integrated singularly in one of four phage attachment site. The fold-change for each reporter (luciferase, GFP, mCherry, mTurquoise) is measured as the reporter signal in the presence of 0.2% of arabinose, divided by the basal activity of the reporter in the absence of arabinose. All data indicate averages from at least two independent biological assays and error bars denote standard deviations. A Student’s t-test (two-tailed, two-sample unequal variance) shows that the mTurquoise signals of the strains carrying the mTurquoise reporter are incompatible with the signals of the strains not carrying the mTurquoise reporter (p-value = 0.003), indicating that the signals are statistically different. The same holds for all other reporters, for which the difference between strains +/− reporter are more evident