Fig. 1

Establishment of the ex vivo whole hemisphere slice model. a Brain hemisphere tissue weight based on slice location (n = 2–4, median with 95% CI). b LDH absorbance variability based on slice location (n = 4, median with 95% CI). c LDH absorbance of slices as a function of slice weight. R: overnight rest; 1D: after 24 h; LR: linear regression. Cellular morphology of d neurons stained with NeuN (green) and e microglia stained with Iba + (green) after 6 h culturing. Slices were additionally stained with the cell nuclei marker, DAPI (blue). Scale bar is 100 μm