Fig. 2

Gene map for targeted Cas9-mediated gene Knock-In. (A) Map of T7-RNAP in BL21(DE3), including LacI, LacO and T7 RNA Polymerase gene. (B) Integration site in BW25113. (C) map of T7-RNAP integration in ideal condition and the location of PCR product (5496 bp) for sequencing