Fig. 6

Confirmation of the efficiency of the T7 expression system in BW25113-T7. (A) Expression of sYFP under control of T7-LacI promoter in different bacterial strains. The fluorescent signal was detected by Multimode Reader (read: 503, 540) and was normalized to the cell density (OD₆₀₀). (B) The biosynthesis of ALA (5-Aminolevulinic Acid) by T7 Expression System in different bacterial strains. These strains were cultured in Medium M9YE. All experiments were performed in triplicates. P values were calculated using Tukey’s multiple comparisons test (*P < 0.05, **P < 0.01, ***P < 0.001)