Fig. 7

Schematic illustration of the MI-IF-RPA system for COVID-19 detection. Samples containing COVID-19 target genes are loaded into the small reservoir (I), where they are isothermally reverse transcribed, amplified, labeled with biotin and FAM, then bound to the anti-FAM antibody. The samples are then mixed (III) with running buffer from the large chamber (II) for capillary diffusion across detection bands in the lateral flow chamber (IV) [26]