Fig. 3

Effect of different Nef contents on MC3T3-E1 cells. The proliferation (CCK-8) of MC3T3-E1 cells on composites of different Nef contents on day 1(A), 3(B), and 7(C). Relative ALP activity of MC3T3-E1 cells on composites of different Nef contents on day 3 (D) and 7 (E). *p < 0.05, **p < 0.01