Fig. 2

Differentially expressed genes are involved in biological processes related to oxygen regulation, metabolism, cell signaling, and inflammation in the middle cerebral artery occlusion (MCAO) model. A The volcano plot showed the differential gene expression in the brain tissue of the MCAO model. The X-axis represents the fold change of the difference MCAO and control group after conversion to log 2, and the Y-axis represents the significance q-value after conversion to -log 10. Red dots represent upregulated genes, green dots represent downregulated genes, and grey dots represent non-changed genes. Gene ontology enrichment analysis highlighted the involvement of the differentially expressed genes in B oxygen regulation, C metabolism, D cell signaling, E inflammatory and immune responses, F cell proliferation, and apoptosis, and G neurological functions. The size of the dots represents the number of genes, and the color of the dots represents the significance of the processes