Fig. 5
From: The lyophilized chloroplasts store synthetic DARPin G3 as bioactive encapsulated organelles
Binding of purified chloroplast-made DARPin G3 from fresh and lyophilized leaves and chloroplasts to HER2 on the SKBR-3 as HER2-positive and MDA-MB-231 as HER2-negative cell lines by immunofluorescent microscopy. Bound DARPin G3 was detected by incubating with an anti-His-tag antibody followed by a FITC-conjugated antibody, which showed HER2-specific targeting on the cell surface. Laser power and gain were kept constant, and brightness and contrast settings were adjusted equally. The scale bar represents 20 μm