Fig. 3
From: Stable, neuron-specific gene expression in the mouse brain
Illustration of the steps involving cryoanesthesia and brain injection. A Stereotaxic instrument used for viral injection. B For cryoanesthesia, the pup is kept on aluminum foil, and not directly in contact with the ice to avoid skin damage. C At P0, lambda (white arrow) and bregma (opposite site) are clearly visible on the surface of the skull. D The ICV injection is performed using the following coordinates ML = ± 1.2, AP = + 0.8, DV = -1.7 mm from the lambda