Fig. 5
From: Stable, neuron-specific gene expression in the mouse brain
eGFP mRNA and protein are highly expressed in the mouse brain. A eGFP mRNA expression levels were determined by qPCR and expressed as % of GAPDH levels; bars represent mean ± SEM, n = 3 mice. B eGFP protein expression was confirmed using immunoblotting on protein samples from mouse brain lysates. Immunoblotting was performed using anti-eGFP (dilution 1:1000) as the primary antibody, and IRDye-800 CW (dilution 1:2000) as the secondary antibody, with TUBB3 (beta 3 tubulin) employed as the internal loading control. CNTL: Control