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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Enhanced therapeutic potential of antibody fragment via IEDDA-mediated site-specific albumin conjugation

Fig. 4

SDS PAGE analysis of conjugation and purification of 4D5scFv-HSA conjugation mixtures and anionic exchange chromatograms. 4D5scFv-G125-HSA is (A) and 4D5scFv-G178-HSA is (B). Lane F1: initial eluted fraction of anionic exchange chromatogram of 4D5scFv-HSA conjugation mixture. Lane F2: final eluted fraction of anionic exchange chromatogram of 4D5scFv-HSA conjugation mixtures (Fig. S3). Lanes FT: flow through fractions of metal affinity chromatograms of 4D5scFv-HSA conjugation mixtures after anionic exchange chromatography. Lanes W: washed fractions of metal affinity chromatograms of 4D5scFv-HSA conjugation mixtures after anionic exchange chromatography. Lanes E: eluted samples of metal affinity chromatograms of 4D5scFv-HSA conjugation mixtures after anionic exchange chromatography

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Journal of Biological Engineering

ISSN: 1754-1611