Fig. 7

LY294002 reversed the stimulating effect of NBP and blocked the PI3K/AKT pathways. A The amount of gene levels of hmTOR, PIK3CA, PIKR1, and PIK3R2 in hBMSCs, were cultivated in culture media for 2 d with or without LY294002 (10 μm) or NBP (4 min). hBMSCs were cultured with or without NBP treatment at 4 min or LY294002 (10 μm); B Results of western blot analysis of PI3K, phosphorylated AKT, and PTEN. C Represents the quantification of band intensity on day 2 were performed. GAPDH was used for the normalization. D Representative images of Ca deposition in hBMSCs via Alizarin Red Staining on day 2 under the light microscope. Scale bars, 100 µm. E Quantified areas of ARS staining measured by ImageJ software. The mean and standard deviation values are used to represent the experimental results. The statistical significance of the data points was assessed using the student’s t-test, and significant differences were indicated by *p < 0.05; **p < 0.01 compared with NC, compared with NBP (4 min)