Fig. 2

AAV9 and mutant AAV infectivity. Hela cells were exposed to AAV9 wild-type (WT) and mutant vectors at a multiplicity of infection (m.o.i.) of 2E5 vg/cell. After 72 hours of infection, the viral genome was quantified by assessing the presence of the CMV promoter (A), and expression levels were determined by measuring GFP transgene expression (B)