Fig. 2

Immunofluorescence staining of F-actin (red), HIF-1α (green), and nucleus (blue) in iPSC-CM cells cultured on a polystyrene plate (control), and PCL and PU nanofibrous mats under normoxia (21% O2), 6 h hypoxia (1% O2) (A), and hypoxia 6 h + 24 h re-oxygenation. (B) The expression of HIF-1α of iPSC-CM cells (C) after hypoxia and (D) after hypoxia with re-oxygenation. *- p < 0.05- statistically significant differences compared to control normoxia, *with line-p < 0.05 statistically significant differences between groups. n > 3. Scale bar 50 μm