Fig. 1
From: Isolation methods and characterization of primary rat neurovascular cells
General overview for primary neurovascular cell isolation. The isolation procedure for astrocytes, pericytes, and endothelial cells, generally follows a similar sequence of five steps: (1) Tissue Extraction; starting with extracting the tissue following euthanasia, (2) Antibiotic Rinse; an introduction to antibiotic media, (3) Cerebral Isolation; by removing the meninges, olfactory bulb, and cerebellum, (4) Using mechanical dissociation techniques, (5) Additional Separations; to start separating specific cell types and (6) Cell enrichment; encouraging specific cell adhesion and proliferation through media components and surface coatings. Steps 1–4 produce a single cell suspension from brain tissue while Steps 5 and 6 produce specific cell types. This figure was created with Biorender.com