Fig. 3

Astrocyte growth in culture: Single cell suspensions are visible (green arrow) even without using enzymatic digestion in the isolation process. At 1 DIV, small cell somas can be seen attached to the bottom of the plate with 1–2 branches beginning to form. By 2 DIV, the first media change is completed as indicated by the red triangle icon. Cell debris from dying non-astrocyte cells are depicted by red arrows. Other non-attached cells can be seen (purple arrow). The blue arrow shows many cell bodies growing in the same area forming snowflake-like clusters. These clusters can be seen extending through culture days. At 5 DIV, cells are passaged, and morphology remains heterogeneous, but does not change with more time in culture. Asterisks indicate timepoints where confocal imaging was completed. Scale bars in all images: 200 μm