Fig. 6
From: Isolation methods and characterization of primary rat neurovascular cells
Immunocytostaining for cell identity. Left: GFAP + astrocytes (green), Iba1 + microglia (pink), and MAP2 + neurons (cyan) are presented in culture at 12 DIV. Middle: NG2 + pericytes (purple) show rhomboid phenotype with cells growing on top of one another in multiple Z planes at 12 DIV. Right: ZO-1 + endothelial tight junctions (red) form between endothelial cells at 5 DIV. All visible nuclei are DAPI+ (blue). Scale bars in all images: 100 μm