Fig. 7
From: Isolation methods and characterization of primary rat neurovascular cells
Presence of microglia, neurons, and oligodendrocytes. (A) Top: GFAP + astrocytes (green), Iba1 + microglia (pink), and MAP2 + neurons (cyan) are presented in culture at 3 DIV and 12 DIV. Microglia are visible in both time points but decrease in percentage and change morphology to become less branched at 12 DIV. Bottom: GFAP + astrocytes (green), Olig2 + oligodendrocytes (red), and MAP2 + neurons (cyan) are present in culture at 3 DIV and 12 DIV. The relative number of Olig2 + cells does not appear to change between timepoints. In both images at 3 DIV, some MAP2 + neurons are visible with the number and size of neurons decreasing by 12 DIV. All visible nuclei are DAPI+ (blue). Scale bars in all images: 50 μm. (B) Glial populations are mostly astrocytes at each time point with the fraction of microglia to total cells decreasing at 10 DIV and 12 DIV and the fraction of oligodendrocytes to astrocytes remaining relatively constant. The DAPI refers to all nuclei that is neither one of the two other cell types analyzed in each group