Fig. 4

Induction of calcification of 2D hVIC cultures in dynamic MPS culture in comparison to static counterparts (chip static control and 96-well format). (A) Calcium concentration was quantified and related to protein concentration after 96-hour incubation in ADGM and (B) in PM and control medium (Friedmann and Dunn`s-test; *p < 0.05; **p < 0.01). (C) Cell culture was evaluated microscopically