Fig. 7

Induction of calcification of 2D hVIC cultures dependent on hypoxic and cell incubator oxygen concentration (19%) in dynamic MPS culture in comparison to static counterparts (chip static control and 96-well format). (A) Calcium concentration was quantified and related to protein concentration after 96-hour incubation in ADGM and control medium (two-way ANOVA and Tukey-test; *p < 0.05; ****p < 0.0001; # p < 0.1). (B) Cell culture was evaluated microscopically