Table 1 Primer sequences used for qRT-PCR
Gene | Primer | Sequence (5’-3’) |
|---|---|---|
Reference genes | ||
18s | forward | TCCGACCATAAACGATGCCGAC |
reverse | GGTGAGGTTTCCCGTGTTGAGT | |
GAPDH | forward | CAAGGGCATCCTGGGCTA |
reverse | CCACCACCCTGTTGCTGTAG | |
HPRT | forward | TGGACAGGACTGAACGTCTT |
reverse | GAGCACACAGAGGGCTACAA | |
PPIA | forward | CATACGGGTCCTGGCATCTT |
reverse | GGTGATCTTCTTGCTGGTCTTG | |
Genes of interest | ||
ACTA2 | forward | TCAATGTCCCAGCCATGTAT |
reverse | CAGCACGATGCCAGTTGT | |
Col1a1 | forward | GCTCCTGGTATTGCTGGTG |
reverse | CCAGGTTCACCGCTGTTAC | |
Col3a1 | forward | TAGGTCCATCTGGTCCTGCT |
reverse | ATCGAAGCCTCTGTGTCCTT | |
EGLN1 | forward | TGAGCAGCATGGACGACCTGAT |
reverse | CGTACATAACCCGTTCCATTGCC | |
EGLN2 | forward | CTGTCTGGTATTTTGATGCCAAGG |
reverse | CGGCTGTGATACAGGTACTTGG | |
EGLN3 | forward | GAACAGGTTATGTTCGCCACGTG |
reverse | CCCTCTGGAAATATCCGCAGGA | |
FN1 | forward | CGGTGGCTGTCAGTCAAAG |
reverse | AAACCTCGGCTTCCTCCATAA | |
Hif1a | forward | CATAAAGTCTGCAACATGGAAGGT |
reverse | ATTTGATGGGTGAGGAATGGGTT | |
Hif2a | forward | AGTTCACCTACTGTGATGACAGA |
reverse | GACCCTTGGTGCACAAGTTC | |
LDHA | forward | AGCCCGATTCCGTTACCT |
reverse | CACCAGCAACATTCATTCCA | |
Runx2 | forward | GGTACCAGATGGGACTGTGG |
reverse | GGTGAAACTCTTGCCTCGTC | |
Sox9 | forward | GAACGCACATCAAGACGGAG |
reverse | AGTTCTGGTGGTCGGTGTAG | |