Fig. 4

Phenotypic assays of KR-overexpressing transformants. Phenotypic comparisons were conducted between 7 homologous recombination transformants at HRsite 2 (KR-HR) and 8 randomly integrated transformants (KR-RI) in Schizochytrium sp. KCTC14661. a Growth curves with box-and-whisker plots. b Glucose consumption profiles with box-and-whisker plots. c Fatty acid methyl ester (FAME) contents shown using box-and-whisker plots. d Profiles of fatty acid compositions displayed using box-and-whisker plots. Each set of 7 KR-HR and 8 KR-RI transformants was cultured in duplicate, and data for KR-HR and KR-RI were averaged. Additional details on the cultivation data of each strain are presented in the Fig. S1-4. Cultivation was carried out in 250 mL baffled flasks containing 50 mL of GYPS medium, maintained at 28 °C, and agitated at 180 rpm. FAME and fatty acid compositions were analyzed at 46 h