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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Probing the orthogonality and robustness of the mammalian RNA-binding protein Musashi-1 in Escherichia coli

Fig. 2

Impact of mutations in MSI-1* on the regulatory activity. a Scheme of the MSI-1* gene showing the different point mutations introduced. b Scheme of the different 5’ UTRs considered (containing RNA motifs). c-e Dynamic ranges of the responses upon induction with IPTG (1 mM) of the different genetic systems. pRM1+ and pREP7 in c), pRM1+ and pREP6 in d), and pRM1+ and pREP6b in e). Error bars correspond to standard deviations (three replicates). AU, arbitrary units. f Relationship between the predicted and experimental fold change for pRM1+ and pREP7. The predicted fold change for pRM1+ and pREP7 is the product of the experimental fold changes for pRM1+ and pREP6 and pRM1+ and pREP6b

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Journal of Biological Engineering

ISSN: 1754-1611