Fig. 3

Evolutionary stability of an engineered circuit involving MSI-1*. a Heatmap of the daily dynamic ranges (average of three replicates) of the responses upon induction with IPTG (1 mM) of the different genetic systems evolved during 7 d (46.5 generations). Data for five lines of cells co-transformed with pRM1+ and pREP7 evolved in a medium without IPTG. On the bottom, scheme of the genetic circuit. Crosses indicate that the genetic element accumulated mutations with time. b Heatmap of the daily dynamic ranges of the responses upon induction with IPTG. Data for five lines of cells co-transformed with pRM1+ and pREP7 evolved in a medium with IPTG. c Heatmap of the daily dynamic ranges of the responses upon induction with IPTG. Data for five lines of cells co-transformed with pRM1 and pREP7 evolved in a medium without IPTG. d Heatmap of the daily dynamic ranges of the responses upon induction with IPTG. Data for five lines of cells co-transformed with pRM1 and pREP7 evolved in a medium with IPTG. AU, arbitrary units