Fig. 3

Effects of cytokine stimulation on microglia secretion. (A) Heatmap representing microglia proteins that change following IFN-γ/TNF-α stimulation (-CTL vs. + CTL). Two-way hierarchical clustering using Ward method. (B) STRING database generated network map of proteins increased (red) and decreased (green) after stimulation (+ CTL) and their predicted functional associations (edges). Thicker and darker edges (line connecting 2 proteins), implies higher level of confidence in the interactions and correlation between the proteins (http://version10.string-db.org/help/faq/). (C) Proteins that are significantly different between controls and arranged in descending order of their number of functional associations (node degrees). p < 0.05 using unpaired t-test with Welch correction for all proteins. (D) Pathways associated with changes in microglia secretion post stimulation