Fig. 8
Microglia proteomic profile shifts after cytokine stimulation and with MSC-EV treatment. (A) Heatmap representing the 2662 proteins identified using LC-MS/MS and clustered based on Euclidian distance across all 3 experimental groups. (B) PCA reveals overall change in protein profile with PC1proteome (34.2% variance) accounting for alternate protein expression and PC2proteome (26.9% variance) accounting for shift in protein expression towards -CTL after MSC-EV treatment. (C) Volcano plot depicting alterations in protein expression upon MSC-EV treatment vs. + CTL. (D) Subset of enriched pathways identified using GSVA analysis. *p < 0.05 conducted using ANOVA followed by Tukey post-hoc test, then Benjamini–Hochberg false discovery rate (FDR) adjustment for multiple comparisons were used. FDR adjusted p-values ≤ 0.05 were considered to be significant