Fig. 2

crRNA19-14 for KRAS^G12X allele detection by CRISPR-Cas13a. a-c Fluorescence ratio (left) and fluorescence level over time (right) in the presence of crRNA19^G12D-14 G12D (a), crRNA19^G12C-14 (b), or crRNA19^G12V-14 (c) and PCR products from matching KRAS mutations or KRAS^WT/WT. a-c The results are presented as the mean ± SEM of n = 6 (a), n = 4 (b) and n = 2 (c) replicates from independent experiments