Fig. 4

Effects of HA nanoparticles on osteoblasts colony formation. (A) Cell colony assay of HOBIT cells after 7 days. (B) Cell colony assay of MG63 cells after 7 days. Analyses of colony formation rates and colony average intensity for HOBIT (C and E) and MG63 D and F). Cells were incubated with particles for 1 day, then detached, counted, and plated; the number of formed cell colonies was counted 7 days after seeding. Individual values are shown as mean ± SD. The resulting p-value is indicated (*p < 0.05; **p < 0.01; ****p < 0.0001)