Fig. 4

A three-layer cascade OR gate. (A) Schematic of a three-layer OR gate. The inputs a (T17) and b (T19) signals are processed by the signal processing layers S17-T18 and S19-T18 for conversion to a common signal T18, which in turn activates the reporter expression in the final reporter layer S18-3WJdB. (B) Characterization of the OR gate with different combinations of input signals a and b. All data shown are n = 3 independent biological replicates. Error bars represent the standard deviation (s.d.) of three biological replicates