Fig. 2

sfGFP and mCardinal production in E. coli, P. putida, and V. natriegens containing the single and dual expression systems. For all samples, the fluorescence mean of sfGFP signal (excitation 485, emission 510) and mCardinal signal (excitation 605, emission 659) was normalized by the cell density (OD₆₀₀). Gene circuits of A σ⁷⁰ V2TcR-sfGFP B σ⁷⁰ V2/3LacI-mCardinal & C σ⁷⁰ V2TcR-sfGFP/σ⁷⁰V3LacI-mCardinal. Normalized sfGFP emission produced by σ⁷⁰ V2TcR-sfGFP in D E. coli F P. putida & H V. natriegens. Normalized mCardinal emission produced by σ⁷⁰ V2/3LacI-mCardinal in E E. coli G P. putida & I V. natriegens. Normalized sfGFP and mCardinal emission produced by the dual expression system σ⁷⁰V2TcR-sfGFP/σ⁷⁰V2/3LacI-mCardinal in J, K E. coli L, M P. putida & N, O V. natriegens. (Error bars are ± S.D., n = 3. P Matrix display of both sfGFP and mCardinal production of individual and concurrent inductions of the dual expression systems