Fig. 3

Assessing potential synergy between lac and tet promoters. Gene circuits of the single expression system A σ⁷⁰V2TcR-mCardinal, σ⁷⁰V2LacI-mCardinal or σ⁷⁰V3LacI-mCardinal & the dual expression system B σ.⁷⁰V2TcR-mCardinal-V2/3LacI-mCardinal. Time course of mCardinal production from the single expression systems in C E. coli E P. putida & G V. natriegens Vs mCardinal production in the double expression system in D E. coli F P. putida & H V. natriegens. For all samples, the fluorescence mean of mCardinal signal (excitation 605 nm, emission 659 nm) was normalized by the cell density (OD₆₀₀). Error bars are ± S.D., n = 3