Fig. 4

Production of lycopene and β-carotene in E. coli, P. putida, and V. natriegens with the single and dual expression systems. Gene circuit of A σ⁷⁰V2TcR-crtEBI and B σ⁷⁰V2TcR-crtEBIY. Production of C lycopene and D β-carotene by the single σ⁷⁰V2TcR expression system in E. coli, P. putida and V. natriegens. Gene circuit of E the dual expression system σ.⁷⁰V2TcR-crtEBI-V2/3LacI-crtY. Production of F lycopene and G β-carotene by the dual expression system in E. coli, P. putida and V. natriegens. Cultures were induced with aTc, IPTG or both inducers at OD₆₀₀. 0.7 for 4 h and terpenoids titers were measured by UHPLC. Error bars are ± S.D., n = 3