Fig. 8

Production of lycopene and β-carotene in E. coli using the single and dual expression systems with THS transformed with pSC101 vector. Architecture of the σ⁷⁰V2TcR σ.⁷⁰V3-2LacI-THS controlling the A LYC operon and B crtEBIY genes. Production of C lycopene and D β-carotene by the single expression systems. E Gene circuit of the dual expression system with THSs F Lycopene and β-carotene production by the dual expression system. Cultures were induced with aTc, IPTG or both inducers at OD₆₀₀. 0.7 for 4 h and terpenoids titers were measured by UHPLC. Error bars are ± S.D., n = 3