Fig. 1

(a) Process overview of mEV isolation from unpasteurized bovine milk (b) NTA particle size distribution of freshly isolated mEVs, reported as mean, mode, standard deviation (SD), sample particle count (Count/mL), and calculated polydispersity index (PDI). Zetasizer obtained zeta potential (Zeta Pot.) and PDI (annotated ZS) (n = 3). (c) TEM image of mEVs (Mean: 126 nm, SD: 42 nm, N = 60 particles measured) with zoomed-in panel of isolated mEVs (d) Calcein-AM loaded mEVs under fluorescent microscopy