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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Stabilizing milk-derived extracellular vesicles (mEVs) through lyophilization: a novel trehalose and tryptophan formulation for maintaining structure and Bioactivity during long-term storage

Fig. 4

(a) TEM images of lyophilized mEV reconstituted in diH2O, (b) NTA particle distribution (mean {black bar}, mode {yellow marker}, STDEV {error bars), PDI, and particle counts. Means were not statistically significant using multiple T tests with Bonferroni Correction (α = 0.05) (c) ECIS assay bioactivity of lyophilized mEV samples stored for 1 month at room temperature in various concentrations of Trehalose (TH), N = 4. ECIS wound recovery was analyzed using multiple T tests with Bonferroni Correction (α = 0.05); there was significantly diminished bioactivity in HEPES-lyophilized samples compared to fresh mEVs and 25mM, 50mM and 75mM TH groups all had statistically greater bioactivity compared to HEPES-lyophilized controls (d) DSC thermal stability of mEV powders N = 1; error bars represent standard error associated with calibration. HEPES blanks were obtained by lyophilizing 5mL of neutral buffer. Pre-SEC mEVs were obtained by lyophilizing 5mL of eluent before concentrating. Thermograms of a few of the samples are available in Figure S7

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Journal of Biological Engineering

ISSN: 1754-1611