Fig. 1

Oxygen transfer rate (OTR) of CHO DP12 cell cultures. Two independent experiments (1 and 2) were performed. For both experiments, a round 96-deep-well microtiter plate and 250 mL shake flasks were inoculated. The µTOM device was used for online monitoring of the microtiter plates (dark red line and circles; light red line and triangles) and the TOM device for the shake flasks (black line and squares; grey line and diamonds). For clarity, only every 24th measuring point over time is marked as a symbol. The microtiter plate experiments were performed in 72 (Experiment 1) and 66 (Experiment 2) replicates and the shake flask experiments in 3 replicates each. For clarity, the low standard deviations are not shown in this figure but can be found in Fig. S2. Culture conditions TOM device: 250 mL glass flasks, temperature (T) = 36.5 °C, shaking frequency (n) = 140 rpm, shaking diameter (d₀) = 50 mm, filling volume (V_L) = 50 mL, 5% CO₂, 70% rel. hum., medium: TCX6D + 8 mM glutamine; starting cell density: 5 × 10⁵ cells mL⁻¹. Culture conditions µTOM device: round 96-deep-well microtiter plate, temperature (T) = 36.5 °C, shaking frequency (n) = 850 rpm, shaking diameter (d₀) = 3 mm, filling volume (V_L) = 1 mL, 5% CO₂, humidified, medium: TCX6D + 8 mM glutamine; starting cell density: 5 × 10⁵ cells mL⁻¹