Fig. 2

Oxygen transfer rate (OTR) of CHO DP12 cell cultures monitored by the µTOM device. A OTR curves of cultivations in a round 96-deep-well plate with different filling volumes (N = 8 for each filling volume). B OTR curves of cultivations in a square 96-deep-well plate with different filling volumes (N = 3 or 6 for each filling volume). For clarity, only every 24th measuring point over time is marked as a symbol. The standard deviations are shown as shaded areas. Culture conditions: temperature (T) = 36.5 °C, shaking frequency (n) = 850 rpm, shaking diameter (d₀) = 3 mm, varying filling volume (V_L), 5% CO₂, humidified, medium: TCX6D + 8 mM glutamine; starting cell density: 5 × 10⁵ cells mL−1