Fig. 4
CHO DP12 cell cultivations in round 96-deep-well plates (dark and light red lines/circles and upward triangle), shake flasks (black and gray lines/squares and diamonds), and a stirred tank reactor (blue line and pentagon). A Depicted is the oxygen transfer rate (OTR). The curves of the microtiter plate and shake flask cultivations are already shown in Fig. 1 and plotted here again for improved comparability. Additionally, the curve of a shake flask cultivation with increased volumetric power input (P/V) from Neuss et al. [34] is shown. The data for the STR are interpolated over 3 h. For original data refer to Fig. S5 B. For clarity, only one measuring point per day is shown. B Displayed are the viable cell density (VCD) and viability for all cultivations. Culture conditions TOM device: 250 mL glass flasks, temperature (T) = 36.5 °C, shaking frequency (n) = 140 rpm, shaking diameter (d0) = 50 mm, filling volume (VL) = 50 mL, 5% CO2, 70% rel. hum., medium: TCX6D + 8 mM glutamine; starting cell density: 5 × 105 cells mL−1. Culture conditions µTOM device: round 96-deep-well microtiter plate, temperature (T) = 36.5 °C, shaking frequency (n) = 850 rpm, shaking diameter (d0) = 3 mm, filling volume (VL) = 1 mL, 5% CO2, humidified, medium: TCX6D + 8 mM glutamine; starting cell density: 5 × 105 cells mL−1. Culture conditions stirred tank reactor: 1.5 L reactor, temperature (T) = 36.5 °C, stirrer speed (n) = 100–250 rpm (Rushton turbine), filling volume (VL) = 600 mL, 5% CO2, aeration = 0.2 vvm (sparged), medium: TCX6D + 8 mM glutamine; starting cell density: 5 × 105 cells mL−1