Fig. 2
From: Engineering Yarrowia lipolytica for the production of β-carotene by carbon and redox rebalancing
Elimination of lycopene inhibition and recovery of production level. A The CRISPR-cas9 system by which Y27R mutation was introduced into the YB4 strain. B Normalized fluorescence intensity of carRP-hrGFP fusion protein expressing strains. The star, with its distinct colors, indicates different codons for the Y27R mutation. The double tilde signs represent the discretization of the x-axis for clear comparison between weak fluorescent strains. C Carotenoid production and specific growth rate of derivative strains. Each plus sign represents a single round of integration of corresponding gene expression cassettes into the genome or deletion of complete CDS from the genome. Error bars represent the standard deviations of three biological replicates. The red dashed line indicates the β-carotene titer (mg/L) of YB4 from Fig. 1B