Skip to main content
Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Engineering probiotic Escherichia coli for inflammation-responsive indoleacetic acid production using RiboJ-enhanced genetic circuits

Fig. 3

Functional characterization of the thiosulfate-inducible IAA biosynthesis. (A and B) IAA titer (mg/L) of the engineered EcN strains harboring (A) pPhsA-IAA6 or (B) pPhsA-IAA7 plasmids in response to various combinations of tryptophan and thiosulfate concentrations. The engineered EcN strains were cultivated in an IAA production medium with various combinations of tryptophan (0, 0.5, 1, and 2 g/L) and thiosulfate (0, 0.04, 0.2, and 1 mM) levels for 24 h at 37 °C. Cell growth and IAA concentrations were measured using a UV-VIS spectrophotometer and HPLC analysis, respectively. Data represent the mean and SD from three biological replicates

Back to article page

Journal of Biological Engineering

ISSN: 1754-1611