Fig. 5

Respiration activity of G. oxydans at varying oxygen limitations. OTR, CTR, RQ and sugar concentrations over time of G. oxydans 621 H ΔhsdR in Jülich-medium containing 80 g/L mannitol as C-source at two different filling volumes (10 mL and 25 mL). Experiments were performed as duplicate measurements in two individual shake flasks. Time on x-Axis is shifted at some curves for clarity: -0.25 h for 10 mL red square, + 0.25 h for 10 mL red triangle. A OTR B CTR C RQ D Measured (square and triangle symbols) and model predicted (solid and dotted lines) sugar concentrations. Model based on total oxygen consumption, total carbon dioxide production and reaction stoichiometry (see Additional file 4); mannitol (measurement: closed squares, model prediction: solid line); fructose (measurement: open triangles, model prediction: dotted line). Sugar measurements are single point measurements. Calculated values are based on average total oxygen consumption and average total carbon dioxide production from two individual shake flasks (see Additional file 3). Operation conditions: n = 350 rpm, d₀ = 50 mm, V_L = 10 and 25 mL, 250 mL shake flask, T = 30 °C, t_meas = 12 min reduced to t_meas = 6 min after 3 h