Fig. 2
From: Modular plasmid design for autonomous multi-protein expression in Escherichia coli
The background expression and dynamic range of A RhaS/RhaBAD, C LacI/Trc, E AraC/AraBAD, G XylS/Pm single-gene cassettes monitored through the fluorescence intensity of mCherry over a 24 h cultivation. The normalised fluorescence of mCherry at 18 h of cultivation was plotted against inducer concentration for B RhaS/RhaBAD, D LacI/Trc, F AraC/AraBAD, H XylS/Pm cassette induction and analysed by one-way ANOVA and Levene’s tests, which results are shown in Table S2. Specific differences between neighbouring groups were tested using Tukey’s post hoc analysis. Error bars represent the standard deviations of 6 individual replicates. Fluorescence values were normalised against the OD600. Plain E. coli BL21 (DE3) strain was used as a control. Abbreviation: ns – not significant